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Modulation of glycolysis induction in primary cultures of rabbit kidney proximal tubule cells: the role of shaking, glucose and insulin.
Author(s) -
Monteil Christelle,
Leclere Caroline,
Dantzer Françoise,
Elkaz Veronika,
Fillastre JeanPaul,
Morin JeanPaul
Publication year - 1993
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.1993.1019
Subject(s) - glycolysis , proximal tubule , insulin , kidney , modulation (music) , primary culture , chemistry , microbiology and biotechnology , endocrinology , rabbit (cipher) , medicine , biology , biochemistry , metabolism , in vitro , physics , statistics , mathematics , acoustics
We have assessed the impact of increasing oxygen availability on cellular phenotype expression of rabbit proximal tubule cells in primary culture developed with variable glucose and/or insulin contents. To mitigate hypoxia at the cell/medium interface, cells were shaken for the whole culture duration and their expressed phenotype was compared with those expressed by static cultures. O 2 and CO 2 tensions were kept constant in the incubator atmosphere. Glycolysis and gluconeogenesis pathways, detoxication system, and mitochondrial, apical and basolateral membrane marker enzyme activities were assessed. This study showed that the induction of glycolysis which appear in primary cultures of proximal tubule cells may be partially prevented by continuously shaking the cultures. This effect was more marked in the presence of glucose, suggesting better substrate oxidation in shaken cultures.