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Failure of centromere separation leads to formation of diplochromosomes in next mitosis in okadaic acid treated HeLa cells.
Author(s) -
Ghosh Sibdas,
Paweletz Neidhard,
Schroeter Dieter
Publication year - 1993
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.1993.1018
Subject(s) - sister chromatids , mitosis , metaphase , anaphase , okadaic acid , centromere , microbiology and biotechnology , hela , chromatid , biology , interphase , sister chromatid exchange , cell cycle , genetics , chemistry , phosphatase , cell , chromosome , phosphorylation , dna , gene
Abstract High concentrations of okadaic acid, sufficient to inhibit phosphatase 1 and 2A activities, induces formation of diplochromosomes in HeLa cells. It has been shown that this is due to a failure of sister chromatid separation in earlier mitosis in the presence of okadaic acid in the medium and not due to bypassing of mitosis (endoreduplication). Moreover, it has been demonstrated that the sister chromatid adherence does not depend on any under‐replicated chromatin segment shared by the sister chromatids which might happen in okadaic acid induced premature mitosis, but due to the failure of the centromeres to separate at metaphase ‐ anaphase transition. The role of phophatase 1 in sister chromatid separation has been discussed