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Stimulation of DNA synthesis in skin fibroblasts by human hepatocyte growth factor/scatter factor.
Author(s) -
Kataoka Hirotoshi,
Gohda Eiichi,
Matsunaga Tetsuhiko,
Ishii Takehisa,
Hara Hiroto,
Yamamoto Itaru
Publication year - 1993
Publication title -
cell biology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.932
H-Index - 77
eISSN - 1095-8355
pISSN - 1065-6995
DOI - 10.1006/cbir.1993.1006
Subject(s) - hepatocyte growth factor , dna synthesis , epidermal growth factor , growth factor , stimulation , endocrinology , human skin , microbiology and biotechnology , medicine , chemistry , monoclonal antibody , dna , insulin like growth factor , thymidine , hepatocyte , fibroblast , recombinant dna , biology , antibody , in vitro , biochemistry , immunology , receptor , gene , genetics
The effect of hepatocyte growth factor/scatter factor (HGF/SF) on the proliferation of human skin fibroblasts was examined. At concentrations above 1.0 ng/ml, both native and recombinant human HGF/SF stimulated the DNA synthesis determined by [ 3 H]thymidine incorporation, which was completely inhibited by an anti‐human HGF/SF monoclonal antibody. The maximal DNA synthesis in the treated cells was nearly twice that in untreated cells. HGF/SF also caused an increase in the labelling index, DNA content and cell number. The effect of HGF/SF was more than additive to the maximal effect of insulin and epidermal growth factor, other mitogens for the fibroblasts. These results indicate that human skin fibroblasts are sensitive to the mitogenic action of HGF/SF.