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Short‐term cold storage of seminal samples of the golden lancehead pitviper ( Bothrops insularis)
Author(s) -
Silva Kalena Barros,
Frediani Mayra Hespanhol,
Angrimani Daniel de Souza Ramos,
Coeti Rafaela Zani,
Pereira Ricardo José Garcia,
Nichi Marcílio,
AlmeidaSantos Selma Maria
Publication year - 2021
Publication title -
zoo biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.5
H-Index - 54
eISSN - 1098-2361
pISSN - 0733-3188
DOI - 10.1002/zoo.21590
Subject(s) - biology , extender , semen , cryopreservation , sperm , artificial insemination , zoology , semen cryopreservation , seasonal breeder , reproductive biology , endangered species , reproduction , ecology , fishery , sperm motility , habitat , anatomy , botany , pregnancy , embryo , materials science , genetics , composite material , embryogenesis , polyurethane
Assisted reproductive technologies such as cooling, freezing, and artificial insemination are essential in conserving genetic diversity of critically endangered reptiles like the golden lancehead pitviper ( Bothrops insularis ). Therefore, we examined viability of semen samples from captive golden lanceheads diluted in coconut‐water based extender over a 48‐h period of cooling (5°C). Semen evaluations were performed immediately after the dilution and at 6, 24, and 48 h, using computer‐assisted sperm analysis and stains to assess plasma membrane and acrosomal status. Our findings showed that the extender and protocol employed here were effective in preserving golden lancehead pitviper spermatozoa for a short periods of time (48 h), allowing semen samples to be transported to distant locations for immediate use without the setbacks of cryopreservation.