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A simplified method for extracting androgens from avian egg yolks
Author(s) -
Kozlowski Corinne P.,
Bauman Joan E.,
Caldwell Hahn D.
Publication year - 2008
Publication title -
zoo biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.5
H-Index - 54
eISSN - 1098-2361
pISSN - 0733-3188
DOI - 10.1002/zoo.20221
Subject(s) - biology , androgen , hormone , extraction (chemistry) , zoology , endocrinology , medicine , chromatography , chemistry
Female birds deposit significant amounts of steroid hormones into the yolks of their eggs. Studies have demonstrated that these hormones, particularly androgens, affect nestling growth and development. In order to measure androgen concentrations in avian egg yolks, most authors follow the extraction methods outlined by Schwabl (1993. Proc. Nat. Acad. Sci. USA 90:11446–11450). We describe a simplified method for extracting androgens from avian egg yolks. Our method, which has been validated through recovery and linearity experiments, consists of a single ethanol precipitation that produces substantially higher recoveries than those reported by Schwabl (1993. Proc. Nat. Acad. Sci. USA 90:11446–11450). Zoo Biol 28:137–143, 2009. © 2008 Wiley‐Liss, Inc.