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Noninvasive semen collection from an adult orangutan
Author(s) -
Vandevoort Catherine A.,
Neville Laurie E.,
Tollner Theodore L.,
Field Leslie P.
Publication year - 1993
Publication title -
zoo biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.5
H-Index - 54
eISSN - 1098-2361
pISSN - 0733-3188
DOI - 10.1002/zoo.1430120303
Subject(s) - biology , semen collection , semen , artificial insemination , sperm , cryopreservation , capacitation , andrology , semen cryopreservation , population , reproductive biology , zoology , anatomy , sperm motility , embryo , genetics , embryogenesis , pregnancy , medicine , demography , sociology
Preservation of the genetic diversity of the captive orangutan, especially the wild‐caught founders, is critical in maintaining a long‐term population in zoological parks. One solution to the problem of maintaining maximum genetic diversity would be to initiate a program of artificial insemination for genetically underrepresented individuals through the banking and interinstitutional use of cryopreserved semen. However, little is known about basic orangutan semen characteristics, and current methodology is inadequate to support such a program. In this paper, we report the results of semen collection from an adult Sumatran orangutan ( Pongo pygmaeus abelli ), using an artificial vagina without anesthesia or electrical stimulation. A total of 27 ejaculates were evaluated during a 1‐year period. The total and liquid volumes of the ejaculates at 1 h following collection were 6.1 ± 0.6 ml and 2.6 ± 0.4 ml, respectively (mean ± SEM). The liquid portion continued to exude semen for 2 h; however, 90% of the motile sperm was exuded within the first 30 min. The total number of sperm in the ejaculate was 164 ± 10 6 ± 16.5, and the percentage of motile cells was 60 ± 2.7%. We conclude that the artificial vagina provides a promising technique for semen collection in the orangutan, and view these results as an initial step in developing methods for in vitro sperm capacitation, sperm cryopreservation, and artificial insemination. © 1993 Wiley‐Liss, Inc.