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In vitro capacitation of Siberian tiger spermatozoa
Author(s) -
Byers A. P.,
Hunter A. G.,
Hensleigh H. C.,
Kreeger T. J.,
Binczik G.,
Reindl N. J.,
Seal U. S.,
Tilson R. L.
Publication year - 1987
Publication title -
zoo biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.5
H-Index - 54
eISSN - 1098-2361
pISSN - 0733-3188
DOI - 10.1002/zoo.1430060404
Subject(s) - capacitation , sperm , biology , hamster , andrology , human fertilization , mesocricetus , in vitro , tiger , golden hamster , sperm motility , anatomy , botany , endocrinology , biochemistry , medicine , computer security , computer science
Three experiments were designed to determine optimum conditions for capacitation of Siberian tiger ( Panthera tigris altaica ) sperm in vitro using the zona‐free hamster egg sperm penetration assay (SPA) as a verification of capacitation. Sperm collected from a 9‐year‐old captive Siberian tiger were subjected to different in vitro washing conditions, preincubation times, and temperatures to induce capacitation. Sperm were able to penetrate zona‐free hamster ova after 2 hours preincubation at 37°C but not at time 0. Preincubation at room temperature was not sufficient to prepare sperm for fertilization. The presence of seminal plasma during the 2‐hour, 37°C preincubation did not affect the ability of tiger sperm to penetrate zona‐free hamster eggs. The SPA can provide a means for evaluation of in vitro capacitation of Siberian tiger sperm.