In vitro  capacitation of Siberian tiger spermatozoa | Zendy

Byers A. P. | Zendy; Hunter A. G. | Zendy; Hensleigh H. C. | Zendy; Kreeger T. J. | Zendy; Binczik G. | Zendy; Reindl N. J. | Zendy; Seal U. S. | Zendy; Tilson R. L. | Zendy

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In vitro capacitation of Siberian tiger spermatozoa

Author(s) -

Byers A. P.,

Hunter A. G.,

Hensleigh H. C.,

Kreeger T. J.,

Binczik G.,

Reindl N. J.,

Seal U. S.,

Tilson R. L.

Publication year - 1987

Publication title -

zoo biology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.5

H-Index - 54

eISSN - 1098-2361

pISSN - 0733-3188

DOI - 10.1002/zoo.1430060404

Subject(s) - capacitation , sperm , biology , hamster , andrology , human fertilization , mesocricetus , in vitro , tiger , golden hamster , sperm motility , anatomy , botany , endocrinology , biochemistry , medicine , computer security , computer science

Three experiments were designed to determine optimum conditions for capacitation of Siberian tiger ( Panthera tigris altaica ) sperm in vitro using the zona‐free hamster egg sperm penetration assay (SPA) as a verification of capacitation. Sperm collected from a 9‐year‐old captive Siberian tiger were subjected to different in vitro washing conditions, preincubation times, and temperatures to induce capacitation. Sperm were able to penetrate zona‐free hamster ova after 2 hours preincubation at 37°C but not at time 0. Preincubation at room temperature was not sufficient to prepare sperm for fertilization. The presence of seminal plasma during the 2‐hour, 37°C preincubation did not affect the ability of tiger sperm to penetrate zona‐free hamster eggs. The SPA can provide a means for evaluation of in vitro capacitation of Siberian tiger sperm.

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