Premium
The development and application of an enzyme immunoassay for urinary estrone conjugates
Author(s) -
Czekala N. M.,
Gallusser S.,
Meier J. E.,
Lasley B. L.
Publication year - 1986
Publication title -
zoo biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.5
H-Index - 54
eISSN - 1098-2361
pISSN - 0733-3188
DOI - 10.1002/zoo.1430050102
Subject(s) - immunoassay , radioimmunoassay , estrone , biology , conjugate , urine , urinary system , chromatography , endocrine system , endocrinology , immunology , hormone , antibody , chemistry , mathematical analysis , mathematics
Abstract An enzyme immunoassay (EIA) for estrone conjugates is described and applied to urine samples from a female Indian rhinoceros, a female gorilla, and a female lion‐tailed macaque. Concomitant measures of estrone conjugates in the same sample are compared to the values obtained with radioimmunoassay. High correlation coefficients for values obtained from each assay indicate that EIA measurements provide information that is comparable to values obtained by radioimmunoassay. EIA methods for urinary steroid conjugates can provide a practical tool to evaluate female reproductive status of zoo species without the need for a traditional endocrine laboratory.