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Birth of a chimpanzee ( Pan troglodytes ) after artificial insemination with cryopreserved epididymal spermatozoa collected postmortem
Author(s) -
Kusunoki Hiroshi,
Daimaru Hideshi,
Minami Shinji,
Nishimoto Shosaku,
Yamane KenIchi,
Fukumoto Yukio
Publication year - 2001
Publication title -
zoo biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.5
H-Index - 54
eISSN - 1098-2361
pISSN - 0733-3188
DOI - 10.1002/zoo.1015
Subject(s) - biology , artificial insemination , cryopreservation , andrology , troglodytes , insemination , gestation , luteinizing hormone , pregnancy , physiology , gynecology , anatomy , endocrinology , embryo , hormone , zoology , medicine , genetics , microbiology and biotechnology
A male chimpanzee ( Pan troglodytes ) suddenly died of acute hemorrhagic enteritis at the age of 18 years. Within 24 hours after its death, a large quantity of spermatozoa of excellent quality was recovered from the distal cauda epididymides and was subsequently cryopreserved. After storage for 67 days, the frozen spermatozoa were thawed and inseminated in an adult, normal cycling, nulliparous female. The optimal day for insemination was estimated by monitoring the swelling of the female’s sex skin and urinary luteinizing hormone concentrations. Pregnancy was confirmed by a urinary chorionic gonadotropin test, and a normal female infant was born after 214 days of gestation. This birth demonstrates that distal cauda epididymal spermatozoa recovered from a dead male can be cryopreserved and successfully inseminated in a female chimpanzee. Zoo Biol 20:135–143, 2001. © 2001 Wiley‐Liss, Inc.