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Molecular sex diagnosis in castoridae
Author(s) -
Kühn Ralph,
Schwab Gerhard,
Schröder Wolfgang,
Rottmann Oswald
Publication year - 2002
Publication title -
zoo biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.5
H-Index - 54
eISSN - 1098-2361
pISSN - 0733-3188
DOI - 10.1002/zoo.10018
Subject(s) - testis determining factor , biology , cytochrome b , polymerase chain reaction , mitochondrial dna , gene , genomic dna , microbiology and biotechnology , genetics , computational biology , y chromosome
Beavers do not exhibit any external sexual trait. Therefore, a simple method was established to differentiate between males and females by verifying the male‐specific SRY gene. Genomic DNA was prepared from hair root cells. Male samples displayed an electrophoretic 157‐bp band derived from the SRY gene after polymerase chain reaction (PCR) amplification. This band is missing in female samples. A 375‐bp fragment of the mitochondrial cytochrome b gene served as internal control and was visible in all samples. The technique can easily be used as a standard monitoring method, and is noninvasive, quick, and reliable. Zoo Biol 21:305–308, 2002. © 2002 Wiley‐Liss, Inc.