Premium
Fluorescence Resonance Energy Transfer between an Anti‐EGFR Antibody and Bi 2 Se 3 /SiO 2 , ZnS/SiO 2 , and ZnSe/SiO 2 Nanomaterials for Biosensor Purposes
Author(s) -
Dolatyari Mahboubeh,
Rostami Ali,
Torabi Pouneh,
Klein Axel
Publication year - 2017
Publication title -
zeitschrift für anorganische und allgemeine chemie
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.354
H-Index - 66
eISSN - 1521-3749
pISSN - 0044-2313
DOI - 10.1002/zaac.201700257
Subject(s) - tetraethyl orthosilicate , fluorescence , nanoparticle , nanomaterials , materials science , förster resonance energy transfer , conjugated system , analytical chemistry (journal) , conjugate , photochemistry , chemistry , nanotechnology , organic chemistry , optics , polymer , mathematical analysis , physics , mathematics , composite material
Bi 2 Se 3 , ZnS, and ZnSe nanoparticles were synthesized and their surface modified using tetraethyl orthosilicate and (3‐aminopropyl) tetraethyl orthosilicate. They were conjugated to an anti‐EGFR antibody. SEM images indicate that the sizes of the modified nanoparticles (NPs) Bi 2 Se 3 /SiO 2 , ZnS/SiO 2 , and ZnSe/SiO 2 are about 20 nm. Förster‐Fluorescence resonance energy transfer (FRET) between the anti‐EGFR antibody and ZnS/SiO 2 , ZnSe/SiO 2 , and Bi 2 Se 3 /SiO 2 nanomaterials was investigated in‐depth showing e.g. that with conjugation of the anti‐EGFR to nanomaterials, fluorescence intensity increases. This increase reaches up to 6.6 times for ZnS/SiO 2 ‐anti‐EGFR conjugate NPs making their sensitivity attractive for biosensor applications. Fluorescence lifetimes were also increased for all conjugated NPs with markedly higher lifetime for Bi 2 Se 3 /SiO 2 compared with ZnS/SiO 2 and ZnSe/SiO 2 .