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Monitoring Cu 2+ ‐Binding to a DNA‐Clip‐phen Conjugate and Metal‐centered Redox Processes by a Fluorescent Reporter Group
Author(s) -
Sprödefeld Arndt,
Kiel Alexander,
Herten DirkPeter,
Krämer Roland
Publication year - 2013
Publication title -
zeitschrift für anorganische und allgemeine chemie
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.354
H-Index - 66
eISSN - 1521-3749
pISSN - 0044-2313
DOI - 10.1002/zaac.201300077
Subject(s) - fluorescence , conjugate , moiety , molecule , dna , chemistry , metal , metal ions in aqueous solution , redox , ligand (biochemistry) , photochemistry , phenanthroline , combinatorial chemistry , crystallography , stereochemistry , inorganic chemistry , biochemistry , organic chemistry , receptor , mathematical analysis , physics , mathematics , quantum mechanics
A DNA conjugate of the bis‐1,10‐phenanthroline ligand Clip‐phen was prepared and combined with a complementary, fluorescent reporter labeled DNA strand. In the resulting ds‐DNA, the fluorescent reporter signals both the binding of Cu 2+ ions to the Clip‐phen moiety and the reduction to Cu + by a change in fluorescence intensity. In preliminary single molecule fluorescence investigations, these processes appear to be observable on the level of individual molecules by single molecule fluorescence spectroscopy, but further validation and optimisation of the system is needed.