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Synthesis, Characterization, Covalent Binding, and Degree of Unwinding of Platinum(II) Bipyridine Complexes
Author(s) -
Patel Mohan,
Gandhi Deepen S.,
Parmar Pradhuman A.,
Bhatt Bhupesh S.,
Patidar Anshul P.
Publication year - 2012
Publication title -
zeitschrift für anorganische und allgemeine chemie
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.354
H-Index - 66
eISSN - 1521-3749
pISSN - 0044-2313
DOI - 10.1002/zaac.201100466
Subject(s) - chemistry , puc19 , covalent bond , 2,2' bipyridine , dna , stereochemistry , bipyridine , binding constant , crystallography , binding site , organic chemistry , plasmid , biochemistry , crystal structure
The complexes [Pt(3′′‐clpbpy)Cl 2 ] ( 1 ) [3′′‐clpbpy = 4‐(3′′‐chlorophenyl)‐6‐phenyl‐2, 2′‐bipyridine], [Pt(4′′‐clpbpy)Cl 2 ] ( 2 ) [4′′‐clpbpy = 4‐(4′′‐chlorophenyl)‐6‐phenyl‐2, 2′‐bipyridine], [Pt(3′′‐brpbpy)Cl 2 ] ( 3 ) [3′′‐brpbpy = 4‐(3′′‐bromophenyl)‐6‐phenyl‐2, 2′‐bipyridine], and [Pt(4′′‐brpbpy)Cl 2 ] ( 4 ) [4′′‐brpbpy = 4‐(4′′‐bromophenyl)‐6‐phenyl‐2, 2′‐bipyridine] were synthesized and characterized. The binding of the complexes with herring sperm DNA (HS DNA) was investigated by absorption titration and viscosity measurements. It was found that the complexes have ability of interaction with DNA by covalent mode. The intrinsic binding constant K b of the complexes with HS DNA is 8.76 × 10 4 ( 1 ), 9.89 ×10 4 ( 2 ), 1.52 × 10 5 ( 3 ), and 2.31 × 10 5 ( 4 ) M –1 . The slight depression in relative specific viscosity was observed, which also attributes to covalent binding of complexes with DNA bases. Gel electrophoresis assay demonstrated the ability of the complexes to unwind negatively supercoiled pUC19 plasmid by 14° ( 1 ), 13° ( 2 ), 13° ( 3 ), and 11° ( 4 ). The in vitro cytotoxic property of the synthesized metal complexes was also carried out against brine shrimp bioassay.