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RHO1 ( YlRHO1 ) is a non‐essential gene in Yarrowia lipolytica and complements rho1Δ lethality in Saccharomyces cerevisiae
Author(s) -
León Maela,
Jaafar Lahcen,
Zueco Jesús
Publication year - 2003
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.961
Subject(s) - yarrowia , saccharomyces cerevisiae , biology , gene , open reading frame , protein subunit , yeast , genbank , orfs , homology (biology) , random hexamer , biochemistry , genetics , peptide sequence
The synthesis of β‐1,3‐glucan, the structural component of the yeast cell wall that gives shape to the cell, occurs at the plasma membrane and is the result of the activity of at least a two‐component complex. Fks1p is the catalytic subunit directly responsible for the synthesis of β‐1,3‐glucan, whilst the second subunit, Rho1p, has a GTP‐dependent regulatory role (Yamochi et al. , 1994). RHO1 has been characterized in Saccharomyces cerevisiae (Yamochi et al. , 1994), and in several other fungal species. In this work, we have used degenerate oligonucleotides derived from the conserved regions of Rho1ps to isolate the RHO1 gene of Yarrowia lipolytica . The gene isolated in this way, which we have named YlRHO1 , encodes a 204 amino acid protein that shows a high degree of homology with other Rho1ps. However, unlike S. cerevisiae , the ylrho1 Δ disruptant strain in Y. lipolytica is viable, although it exhibits an increased sensitivity to Calcofluor white and Congo red. Also, YlRHO1 complements rho1 lethality in S. cerevisiae at both 28° C and 37° C. The complete sequence of YlRHO1 can be obtained from GenBank under Accession No. AF279915. Copyright © 2003 John Wiley & Sons, Ltd.