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Molecular cloning and DNA analysis of a gene encoding α mating pheromone from the yeast Saccharomyces naganishii
Author(s) -
Kodama Takuya,
Hisatomi Taisuke,
Kanemura Tomokazu,
Mokubo Ken,
Tsuboi Michio
Publication year - 2002
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.939
Subject(s) - biology , pheromone , gene , genetics , signal peptide , molecular cloning , peptide sequence , mating , nucleic acid sequence , microbiology and biotechnology
A DNA fragment encoding the precursor peptide for α mating pheromone was isolated from the S. naganishii genome based on the amino acid sequence of the mature pheromone. The precursor peptide contains three copies of the pheromone. Hydrophobicity analysis of the precursor peptide revealed an N‐terminal signal sequence for translocation into the lumen of the endoplasmic reticulum and several signals for a series of secretion‐related processes. However, upstream regulatory sequences necessary for expression of the S. cerevisiae α mating pheromone gene were not found, suggesting the divergence of systems that regulate α mating pheromone gene expression in S. naganishii and S. cerevisiae . Hybridization of a probe corresponding to the S. naganishii α mating pheromone nucleotide sequence to S. naganishii chromosomal DNA revealed a single gene located on either chromosome VI or VII. The S. naganishii α mating pheromone sequence has been deposited in the DDBJ/EMBL/GenBank data library under Accession No. AB086431. Copyright © 2002 John Wiley & Sons, Ltd.