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Isolation and characterization of the gene URA3 encoding the orotidine‐5′‐phosphate decarboxylase from Torulaspora delbrueckii
Author(s) -
HernandezLopez Maria Jose,
Prieto Jose Antonio,
RandezGil Francisca
Publication year - 2002
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.929
Subject(s) - ura3 , biology , genetics , torulaspora delbrueckii , complementation , saccharomyces cerevisiae , gene , microbiology and biotechnology , mutant , saccharomyces
A DNA fragment containing the URA3 gene from Torulaspora delbrueckii was isolated by complementation cloning in Saccharomyces cerevisiae . DNA sequence analysis revealed the presence of an ORF of 795 bp, encoding a 264 amino acid protein, which shares a high similarity to the Saccharomycetaceae Ura3 proteins. Furthermore, the cloned ORF fully complemented the ura3 mutation of S. cerevisiae , confirming that it encodes for the TdUra3 protein. The GeneBank Accession No. for TdURA3 is AF518402. Copyright © 2002 John Wiley & Sons, Ltd.