Isolation and characterization of the gene  URA3  encoding the orotidine‐5′‐phosphate decarboxylase from  Torulaspora delbrueckii | Zendy

HernandezLopez Maria Jose | Zendy; Prieto Jose Antonio | Zendy; RandezGil Francisca | Zendy

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Isolation and characterization of the gene URA3 encoding the orotidine‐5′‐phosphate decarboxylase from Torulaspora delbrueckii

Author(s) -

HernandezLopez Maria Jose,

Prieto Jose Antonio,

RandezGil Francisca

Publication year - 2002

Publication title -

yeast

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.923

H-Index - 102

eISSN - 1097-0061

pISSN - 0749-503X

DOI - 10.1002/yea.929

Subject(s) - ura3 , biology , genetics , torulaspora delbrueckii , complementation , saccharomyces cerevisiae , gene , microbiology and biotechnology , mutant , saccharomyces

A DNA fragment containing the URA3 gene from Torulaspora delbrueckii was isolated by complementation cloning in Saccharomyces cerevisiae . DNA sequence analysis revealed the presence of an ORF of 795 bp, encoding a 264 amino acid protein, which shares a high similarity to the Saccharomycetaceae Ura3 proteins. Furthermore, the cloned ORF fully complemented the ura3 mutation of S. cerevisiae , confirming that it encodes for the TdUra3 protein. The GeneBank Accession No. for TdURA3 is AF518402. Copyright © 2002 John Wiley & Sons, Ltd.

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