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Proteins interacting with Lin1p, a putative link between chromosome segregation, mRNA splicing and DNA replication in Saccharomyces cerevisiae
Author(s) -
Bialkowska Agnieszka,
Kurlandzka Anna
Publication year - 2002
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.919
Subject(s) - biology , establishment of sister chromatid cohesion , genetics , cohesin , rna splicing , chromosome segregation , dna replication , saccharomyces cerevisiae , premature chromosome condensation , control of chromosome duplication , mitosis , chromosome , dna , microbiology and biotechnology , gene , rna
Proteins involved in chromosome segregation during mitosis are likely to participate in other cell cycle‐coordinated processes. Using a two‐hybrid screen we identified a novel nuclear protein, Lin1, interacting with Irr1p/Scc3p, a component of the cohesin complex. The second round of two‐hybrid assay with Lin1p as the bait resulted in the identification of six proteins: Prp8, Slx5, Siz2, Wss1, Rfc1 and YIL149w. These proteins have previously been shown to participate in mRNA splicing, DNA replication, chromosome condensation, chromatid separation and alternative cohesion. We propose that Lin1p may constitute a link among these processes. Copyright © 2002 John Wiley & Sons, Ltd.