Simultaneous determination of potassium and rubidium content in yeast | Zendy

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Simultaneous determination of potassium and rubidium content in yeast

Author(s) -

Mulet Jose M.,

Serrano Ramón

Publication year - 2002

Publication title -

yeast

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.923

H-Index - 102

eISSN - 1097-0061

pISSN - 0749-503X

DOI - 10.1002/yea.909

Subject(s) - rubidium , potassium , yeast , atomic absorption spectroscopy , biology , isotopes of potassium , isotope , radiochemistry , absorption (acoustics) , biochemistry , chemistry , materials science , physics , organic chemistry , quantum mechanics , composite material

Rubidium is widely used as a potassium analogue in transport studies in yeast and other organisms. As rubidium (potassium) uptake is modulated by the internal potassium concentration, it is often necessary to determine both Rb + and K + concentrations in the same cell extract. Current methods based on atomic absorption/emission spectroscopy require separate analysis for each cation. Alternatively, unsafe radioactive isotopes can be used. Here we report a convenient, non‐radioactive, HPLC/conductivity‐based method that allows a complete analysis of both cations with a single injection from a cell extract. The increase in Rb + uptake during K + starvation in yeast is easily demonstrated with this method. Copyright © 2002 John Wiley & Sons, Ltd.

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