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Simultaneous determination of potassium and rubidium content in yeast
Author(s) -
Mulet Jose M.,
Serrano Ramón
Publication year - 2002
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.909
Subject(s) - rubidium , potassium , yeast , atomic absorption spectroscopy , biology , isotopes of potassium , isotope , radiochemistry , absorption (acoustics) , biochemistry , chemistry , materials science , physics , organic chemistry , quantum mechanics , composite material
Rubidium is widely used as a potassium analogue in transport studies in yeast and other organisms. As rubidium (potassium) uptake is modulated by the internal potassium concentration, it is often necessary to determine both Rb + and K + concentrations in the same cell extract. Current methods based on atomic absorption/emission spectroscopy require separate analysis for each cation. Alternatively, unsafe radioactive isotopes can be used. Here we report a convenient, non‐radioactive, HPLC/conductivity‐based method that allows a complete analysis of both cations with a single injection from a cell extract. The increase in Rb + uptake during K + starvation in yeast is easily demonstrated with this method. Copyright © 2002 John Wiley & Sons, Ltd.