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Molecular cloning and sequence analysis of the Zygosaccharomyces bailii HIS 3 gene encoding the imidazole glycerolphosphate dehydratase
Author(s) -
Branduardi Paola
Publication year - 2002
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.907
Subject(s) - biology , saccharomyces cerevisiae , complementation , homology (biology) , genetics , gene , nucleic acid sequence , yeast , complementary dna , molecular cloning , mutant
Zygosaccharomyces bailii is a spoilage yeast belonging to the Zygosaccharomyces genus. In recent years these yeasts, due to their exceptional resistance to several stresses, have become more and more interesting as model organisms to study the molecular basis of the said resistance. A Z. bailii cDNA library has been built and the 672 bp nucleotide sequence coding for the HIS 3 gene was cloned by complementation of a Saccharomyces cerevisiae his3 mutant strain. The deduced 223 amino acid sequence shares a high degree of homology with His3p homologues in other non‐conventional yeast species. The GeneBank Accession No. is AY050224. Copyright © 2002 John Wiley & Sons, Ltd.