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Dissection of the promoter of the HAP4 gene in S. cerevisiae unveils a complex regulatory framework of transcriptional regulation
Author(s) -
Brons Janynke F.,
de Jong Marian,
Valens Michèle,
Grivell Leslie A.,
BolotinFukuhara Monique,
Blom Jolanda
Publication year - 2002
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.886
Subject(s) - biology , gene , protein subunit , transcriptional regulation , activator (genetics) , promoter , transcription (linguistics) , regulatory sequence , microbiology and biotechnology , regulation of gene expression , function (biology) , gene expression , genetics , linguistics , philosophy
In S. cerevisiae , the heteromeric Hap2/3/4/5 complex is necessary for induced transcription of a large number of genes involved in oxidative metabolism on non‐fermentable carbon sources. The Hap4p subunit is the activator subunit and at the same time also the regulatory part of the complex, since it is the only one whose level is regulated by carbon source itself. HAP4 promoter analysis shows a 265 bp activating region at position −1006/−741 bp upstream of the ATG start codon. Specific and differential protein‐binding to a 30 nt CSRE‐like sequence within this region was observed with extracts from repressing and inducing carbon sources. Carbon source‐dependent activation mediated by the 265 bp fragment, as well as protein binding to the 30 nt CSRE‐like region, is dependent on the presence of CAT8 function, unveiling a complex framework by which the expression of the HAP4 gene is coordinated. Copyright © 2002 John Wiley & Sons, Ltd.