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Marker construction and cloning of a cut1 ‐like sequence with ARS activity in the fission yeast Schizosaccharomyces japonicus
Author(s) -
Bozsik Aniko,
Szilagyi Z.,
Benko Z.,
Sipiczki M.
Publication year - 2002
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.853
Subject(s) - schizosaccharomyces pombe , biology , complementation , genetics , schizosaccharomyces , auxotrophy , saccharomyces cerevisiae , shuttle vector , plasmid , cloning (programming) , gene , aspergillus nidulans , ploidy , mutant , recombinant dna , vector (molecular biology) , computer science , programming language
The dimorphic fission yeast Schizosaccharomyces japonicus has proved to be an excellent experimental model for the investigation of the eukaryotic cell. Here we show that it has a haplontic life cycle, in which the diploid phase is confined to the zygote. To make it amenable to genetic and molecular analysis, we generated genetic markers and cloned a genomic sequence which acts as ars when integrated into a plasmid. Diploids suitable for testing complementation and recombination between markers can be formed by protoplast fusion. The complementation tests and the recombination frequencies determined in octads of spores identified 28 non‐allelic groups (genes) of mutations of the auxotrophic and mycelium‐negative mutants. Two groups of linked markers were also identified. The cloned fragment, which expresses ars activity, encodes a putative amino acid sequence highly similar to a conserved domain of proteins Cut1 ( Schizosaccharomyces pombe ), BimB ( Aspergillus nidulans ) and Esp1 ( Saccharomyces cerevisiae ). Copyright © 2002 John Wiley & Sons, Ltd.