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The mei3 region of the Schizosaccharomyces pombe genome
Author(s) -
Aves Stephen J.,
Hunt Cherryl,
Xiang Zheng,
Lyne Michael H.,
Wood Valerie,
Rajandream MarieAdèle,
Skelton Jason,
Churcher Carol M.,
Warren Timothy,
Harris David,
Gwilliam Rhian,
Barrell Barclay G.
Publication year - 2002
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.851
Subject(s) - biology , schizosaccharomyces pombe , genetics , orfs , gene , eif4ebp1 , microbiology and biotechnology , open reading frame , peptide sequence , saccharomyces cerevisiae , ribosome , rna
Expression of the mei3 gene is sufficient to induce meiosis in the fission yeast Schizosaccharomyces pombe . The mei3 gene is located 0.64 Mb from the telomere of the left arm of Sz. pombe chromosome II. We have sequenced and analysed 107 kb of DNA from the mei3 genomic region. The sequence includes 14 known genes ( bag1‐B, csh3, dps1, gpt1, mei3, mfm3, pac1, prp31 , rpl38‐1, rpn3, rti1, spa1, spm1 and ubc4 ) and 26 other open reading frames (ORFs) longer than 100 codons: a density of one protein‐coding gene per 2.7 kb. Twenty‐one of the 40 ORFs (53%) have introns. In addition there is one lone Tf1 transposon long terminal repeat (LTR), tRNA Trp and tRNA Ser genes and a 5S rRNA gene. 14 of the novel ORFs show sequence similarities which suggest functions of their products, including a coatomer α‐subunit, a catechol O ‐methyltransferase, protein kinase, asparagine synthetase, zinc metalloprotease, acetyltransferase, phosphatidylinositol 4‐kinase, inositol polyphosphate phosphatase, GTPase‐activating protein, permease, pre‐mRNA splicing factor, 20S proteasome component and a thioredoxin‐like protein. One predicted protein has similarity to the human Cockayne syndrome protein CSA and one with human GTPase XPA binding protein XAB1. Three ORFs are likely to code for proteins because they have sequence similarity with hypothetical proteins, three encode predicted coiled‐coil proteins and four are sequence orphans. The sequence is derived from cosmid clones c4664, c119 and c557 and has been submitted to the EMBL database under Accession Nos AL591302, AL022117, AL110506 and AL023634 (part). Copyright © 2002 John Wiley & Sons, Ltd.