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Molecular and functional analysis of a MIG1 homologue from the yeast Schwanniomyces occidentalis
Author(s) -
Carmona Teresa A.,
Barrado Patricia,
Jiménez Antonio,
Fernández Lobato María
Publication year - 2002
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.846
Subject(s) - biology , saccharomyces cerevisiae , genetics , open reading frame , genomic dna , yeast , genomic library , gene , dna , zinc finger , repressor , microbiology and biotechnology , peptide sequence , gene expression , transcription factor
A putative glucose repressor MIG1 ‐homologue ( SoMIG1 ) was isolated from the amylolytic yeast Schwanniomyces occidentalis . Degenerate primers were designed from the conserved zinc finger regions of Mig1 and CreA proteins from different organisms. PCR using these primers and S. occidentalis genomic DNA as template yielded a single 128 bp product. This fragment was used as a DNA probe to screen a S. occidentalis genomic library. Analysis of the positive clones led to the isolation by PCR of a DNA fragment, which contained an open reading frame (ORF) that would encode a 458 amino acid polypeptide. The DNA binding and effector domains of this putative protein showed an identity of 71% and 15%, respectively, to those of the Mig1 protein from Saccharomyces cerevisiae . The SoMIG1 gene complemented a mig1 mutant of this yeast, which suggests that in S. occidentalis SoMIG1 is a glucose repressor. The Accession No. is AJ417892. Copyright © 2002 John Wiley & Sons, Ltd.