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SRC1 : an intron‐containing yeast gene involved in sister chromatid segregation
Author(s) -
RodríguezNavarro Susana,
Carlos Igual J.,
PérezOrtín José E.
Publication year - 2001
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.803
Subject(s) - biology , genetics , intron , gene , saccharomyces cerevisiae , rna splicing , anaphase , sister chromatids , open reading frame , mutant , meiosis , cell cycle , chromosome , peptide sequence , rna
Analysis of a three‐member gene family in the yeast Saccharomyces cerevisiae has allowed the discovery of a new gene that comprises two contiguous open reading frames previously annotated as YML034w and YML033w. The gene contains a small intron with two alternative 5′ splicing sites. It is specifically transcribed during G 2 /M in the cell cycle and after several hours of meiosis induction. Splicing of the mRNA is partially dependent on NAM8 but does not vary during meiosis or the cell cycle. Deletion of the gene induces a shortening of the anaphase and aggravates the phenotype of scc1 and esp1 conditional mutants, which suggests a direct role of the protein in sister chromatid separation. Copyright © 2002 John Wiley & Sons, Ltd.