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Sulphur amino acid synthesis in Schizosaccharomyces pombe represents a specific variant of sulphur metabolism in fungi
Author(s) -
Brzywczy Jerzy,
Sieńko Marzena,
Kucharska Agnieszka,
Paszewski Andrzej
Publication year - 2001
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.798
Subject(s) - cystathionine beta synthase , biology , aspergillus nidulans , methionine , biochemistry , sulfur metabolism , cysteine , methionine synthase , schizosaccharomyces pombe , saccharomyces cerevisiae , homocysteine , auxotrophy , schizosaccharomyces , lyase , enzyme , yeast , gene , amino acid , mutant
Schizosaccharomyces pombe , in contrast to Saccharomyces cerevisiae and Aspergillus nidulans , lacks cystathionine β‐synthase and cystathionine γ‐lyase, two enzymes in the pathway from methionine to cysteine. As a consequence, methionine cannot serve as an efficient sulphur source for the fungus and does not bring about repression of sulphur assimilation, which is under control of the cysteine‐mediated sulphur metabolite repression system. This system operates at the transcriptional level, as was shown for the homocysteine synthase encoding gene. Our results corroborate the growing evidence that cysteine is the major low‐molecular‐weight effector in the regulation of sulphur metabolism in bacteria, fungi and plants. The Sz. pombe homocysteine synthase gene sequence was submitted to GenBank under Accession No. AF012876. Copyright © 2002 John Wiley & Sons, Ltd.