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Expression levels of transdominant peptides and proteins in Saccharomyces cerevisiae
Author(s) -
Sandrock Tanya,
Poritz Mark,
Kim Marianne,
Feldhaus Michael J.,
Roth Bruce,
Caponigro Giordano,
Kamb Alexander
Publication year - 2001
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.795
Subject(s) - biology , saccharomyces cerevisiae , penetrance , scaffold protein , protein expression , genetics , computational biology , microbiology and biotechnology , gene , signal transduction , phenotype
Abstract From libraries of peptides and protein fragments, several inhibitors that block pheromone response in Saccharomyces cerevisiae have been isolated previously. In many cases, the inhibitors are displayed as part of a scaffold, such as green fluorescent protein. Each of the inhibitors has a characteristic physiological strength or genetic penetrance. In this report, the roles of expression level and display scaffold on the activities of a subset of pheromone‐response pathway inhibitors were examined. Special consideration was given to the relationship between expression levels of specific inhibitors, which may exceed 50 µ M in some instances, and penetrance. Copyright © 2002 John Wiley & Sons, Ltd.