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Isolation and study of KlLSM4 , a Kluyveromyces lactis gene homologous to the essential gene LSM4 of Saccharomyces cerevisiae
Author(s) -
Mazzoni Cristina,
Falcone Claudio
Publication year - 2001
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.772
Subject(s) - kluyveromyces lactis , biology , saccharomyces cerevisiae , gene , kluyveromyces , mutant , genetics , rna splicing , rna
We have isolated the KlLSM4 gene as a multicopy suppressor of a Kluyveromyces lactis mutant which shows a rag − phenotype ( r esistance to a ntimycin A on g lucose). This gene is homologous to the ScLSM4 of Saccharomyces cerevisiae , which codes for an essential 187 amino acid protein containing Sm‐like domains. These motifs are present in the evolutionarily conserved family of the Sm‐like proteins, which are involved in a large number of cellular processes, including pre‐mRNA splicing and mRNA decapping. We demonstrated that the first 72 amino acids of KlLsm4p, which contain the Sm‐like domains, can restore cell viability in both K. lactis and S. cerevisiae cells lacking the wild‐type protein. However, the absence of the carboxy‐terminal region resulted in a remarkable loss of cell viability in the stationary phase. The KlLSM4 sequence has been deposited in the EMBL Data library under Accession No. AJ311719. Copyright © 2001 John Wiley & Sons, Ltd.