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The sequence of a 15 769 bp segment of Pichia anomala identifies the SEC61 and FBP1 genes and five new open reading frames
Author(s) -
Ruíz Teresa,
Sánchez Manuel,
De la Rosa José M.,
Rodríguez Luis,
Domínguez Angel
Publication year - 2001
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.766
Subject(s) - orfs , biology , genetics , open reading frame , gene , homology (biology) , nucleic acid sequence , saccharomyces cerevisiae , genbank , peptide sequence
We have determined the sequence of a 15 769 bp DNA segment of Pichia anomala . The sequence contains seven complete open reading frames (ORFs) longer than 100 amino acids and a putative tRNA gene. Two of the ORFs code for the well‐characterized genes SEC61 (which codes for the core subunit of the ER translocation complex) and FBP1 (encoding fructose‐1,6‐bisphosphatase). A gene coding for a protein similar to S. cerevisiae YDL054c was found between the two genes. These three genes show a different organization (intermingled triples) in three yeast species: Saccharomyces cerevisiae , Candida albicans and P. anomala . Two out of the four remaining ORFs show weak homology with different proteins from other species and the other two show non‐significant similarity with previously sequenced genes. The nucleotide sequence has been submitted to the EMBL database under Accession No. AJ306295. Copyright © 2001 John Wiley & Sons, Ltd.