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14‐3‐3 Proteins are essential for regulation of RTG3‐dependent transcription in Saccharomyces cerevisiae
Author(s) -
van Heusden G. Paul H.,
Steensma H. Yde
Publication year - 2001
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.765
Subject(s) - biology , saccharomyces cerevisiae , mutant , gene , yeast , transcription factor , genetics , transcription (linguistics) , phenotype , microbiology and biotechnology , linguistics , philosophy
Abstract 14‐3‐3 proteins comprise a family of highly conserved proteins that bind more than 60 different, mostly phosphorylated, proteins. The yeast Saccharomyces cerevisiae has two genes, BMH1 and BMH2 , encoding 14‐3‐3 proteins. Disruption of both genes together is lethal. In this study we constructed a mutant with a single, temperature‐sensitive bmh allele. Recessive mutations in SIN4 and RTG3 can suppress the temperature‐sensitive phenotype of this mutant. These genes encode a global transcriptional regulator and a basic helix–loop–helix transcription factor, respectively. The yeast 14‐3‐3 proteins were shown to bind to the Rtg3 protein. Overexpression of RTG3 is lethal even in wild‐type cells. These genetic and biochemical data are consistent with a model in which the 14‐3‐3 proteins are required to keep the Rtg3 protein in an inactive state, which is (one of) the essential function(s) of the 14‐3‐3 proteins. Copyright © 2001 John Wiley & Sons, Ltd.