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Genetic analysis of TAF68/61 reveals links to cell cycle regulators
Author(s) -
Reese Joseph C.,
Green Michael R.
Publication year - 2001
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.761
Subject(s) - biology , cyclin dependent kinase 1 , cell cycle , mutant , genetics , mitosis , gene , phenotype , genetic screen , allele , cell cycle checkpoint , suppressor , synthetic lethality , mutation , microbiology and biotechnology
In yeast, inactivation of certain TBP‐associated factors (TAF II s) results in arrest at specific stages of the cell cycle. In some cases, cell cycle arrest is not observed because overlapping defects in other cellular processes precludes the manifestation of an arrest phenotype. In the latter situation, genetic analysis has the potential to reveal the involvement of TAF II s in cell cycle regulation. In this report, a temperature‐sensitive mutant of TAF68/61 was used to screen for high‐copy dosage suppressors of its growth defect. Ten genes were isolated: T AF s uppressor g enes, TSGs 1–10. Remarkably, most TSGs have either a genetic or a direct link to control of the G 2 /M transition. Moreover, eight of the 10 TSGs can suppress a CDC28 mutant specifically defective for mitosis ( cdc28‐1N ) but not an allele defective for passage through start. The identification of these genes as suppressors of cdc28‐1N has identified four unreported suppressors of this allele. Moreover, synthetic lethality is observed between taf68‐9 and cdc28‐1N . The isolation of multiple genes involved in the control of a specific phase of the cell cycle argue that the arrest phenotypes of certain TAF II mutants reflect their role in specifically regulating cell cycle functions. Copyright © 2001 John Wiley & Sons, Ltd.