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The centromere‐binding factor Cbf1p from Candida albicans complements the methionine auxotrophic phenotype of Saccharomyces cerevisiae
Author(s) -
Eck Raimund,
Stoyan Tanja,
Künkel Waldemar
Publication year - 2001
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.757
Subject(s) - biology , saccharomyces cerevisiae , auxotrophy , candida albicans , gene , genetics , plasmid , centromere , open reading frame , methionine , microbiology and biotechnology , mutant , chromosome , amino acid , peptide sequence
A gene encoding the centromere binding factor 1 (Cbf1p) of the human pathogenic yeast Candida albicans was cloned and characterized. An open reading‐frame was detected which encoded a 223 amino acid protein with a calculated molecular weight of 25.8 kDa and a relative isoelectric point of 5.55. It shares 39% overall amino acid sequence identity with Saccharomyces cerevisiae Cbf1p. We localized the CaCBF1 gene on chromosome 4. Southern analysis indicated that CaCBF1 is probably present as a single copy gene per haploid genome. The CaCBF1 gene under the control of its own promoter was able to complement the methionine auxotrophic growth, the increased mitotic instability of CEN plasmids and the slow growth of a Saccharomyces cerevisiae cbf1Δ mutant strain. The GenBank Accession No. for this gene is AJ303083. Copyright © 2001 John Wiley & Sons, Ltd.