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Molecular cloning of the RPS0 gene from Candida tropicalis
Author(s) -
Baquero Claudia,
Montero Mercedes,
Sentandreu Rafael,
Valentin Eulogio
Publication year - 2001
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.749
Subject(s) - biology , candida tropicalis , neurospora crassa , schizosaccharomyces pombe , gene , genetics , candida albicans , saccharomyces cerevisiae , ribosomal protein , homology (biology) , ura3 , molecular cloning , microbiology and biotechnology , protein subunit , peptide sequence , ribosome , rna , mutant
The Saccharomyces cerevisiae RPS0 A and B genes encode proteins essential for maturation of the 40S ribosomal subunit precursors. We have isolated a homologue of the RPS0 gene from Candida tropicalis , which we named CtRPS0 . The C. tropicalis RPS0 encodes a protein of 261 amino acid residues with a predicted molecular weight of 28.65 kDa and an isoelectric point of 4.79. CtRps0p displays significant amino acid sequence homology with Rps0p from C. albicans, S. cerevisiae, Neurospora crassa, Schizosaccharomyces pombe, Pneumocystis carinii and higher organisms, such as human, mouse and rat. CtRPS0 on a high copy number vector can complement the lethal phenotype linked to the disruption of both RPS0 genes in S. cerevisiae . Southern blot analysis suggests that CtRPS0 is present at a single locus within the C. tropicalis genome. The nucleotide sequence of the CtRPS0 has been deposited at the EMBL data library under Accession No AJ278686. Copyright © 2001 John Wiley & Sons, Ltd.