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Characterization of a Schizosaccharomyces pombe mutant deficient in UDP‐galactose transport activity
Author(s) -
Tanaka Naotaka,
Konomi Mami,
Osumi Masako,
Takegawa Kaoru
Publication year - 2001
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.740
Subject(s) - schizosaccharomyces pombe , hygromycin b , biology , galactose , yeast , saccharomyces cerevisiae , mannose , biochemistry , schizosaccharomyces , cell wall , mutant , gene
In fission yeast, Schizosaccharomyces pombe , the carbohydrate components of the cell wall consist of galactomannan, unlike in Saccharomyces cerevisiae . We previously found that the disruption of gms1 + , a gene encoding the UDP‐galactose transporter required for the synthesis of galactomannan, led to the complete defect of cell surface galactosylation in Sz. pombe . The Δ gms1 strain is therefore useful for the analysis of physiological properties of galactose residues in Sz. pombe . The deletion strain of gms1 + was viable; however, itshowed an aberrant cell morphology and increased sensitivities to digestion with β‐glucanase and to various drugs, such as hygromycin B, sodium orthovanadate and Calcofluor white. A reduction of galactomannan layers of the cell wall in the Δ gms1 strain was observed by scanning and transmission electron microscopic analyses. The addition of osmotic stabilizer suppressed the morphologic defect of the Δ gms1 cells, while other phenotypes were weakly suppressed. The Δ gms1 ( h 90 ) strain was incapable of sexual conjugation during nutritional starvation. These results suggest that the cell surface galactosylation is required not only for non‐sexual flocculation but also for sexual conjugation in Sz. pombe . Copyright © 2001 John Wiley & Sons, Ltd.