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The GDI1 genes from Kluyveromyces lactis and Pichia pastoris : cloning and functional expression in Saccharomyces cerevisiae
Author(s) -
Brummer Mirko H.,
Richard Peter,
Sundqvist Lena,
Väänänen Ritva,
Keränen Sirkka
Publication year - 2001
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.736
Subject(s) - kluyveromyces lactis , biology , pichia pastoris , saccharomyces cerevisiae , genetics , genomic dna , gene , homology (biology) , genomic library , kluyveromyces , yeast , microbiology and biotechnology , peptide sequence , recombinant dna
The nucleotide sequences of 2.8 kb and 2.9 kb fragments containing the Kluyveromyces lactis and Pichia pastoris GDI1 genes, respectively, were determined. K. lactis GDI1 was found during sequencing of a genomic library clone, whereas the P. pastoris GDI1 was obtained from a genomic library by complementing a Saccharomyces cerevisiae sec19‐1 mutant strain. The sequenced DNA fragments contain open reading frames of 1338 bp ( K.lactis ) and 1344 bp ( P. pastoris ), coding for polypeptides of 445 and 447 residues, respectively. Both sequences fully complement the S. cerevisiae sec19‐1 mutation. They have high degrees of homology with known GDP dissociation inhibitors from yeast species and other eukaryotes. The GenBank Accession Nos of the sequences are AF255332 ( K.lactis GDI1 ) and AY007574 ( P. pastoris GDI1 ). Copyright © 2001 John Wiley & Sons, Ltd.