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Isolation and sequence analysis of the gene encoding triose phosphate isomerase from Zygosaccharomyces bailii
Author(s) -
Merico Annamaria,
Rodrigues Fernando,
CôrteReal Manuela,
Porro Danilo,
Maria Ranzi Bianca,
Compagno Concetta
Publication year - 2001
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.727
Subject(s) - biology , triosephosphate isomerase , genbank , open reading frame , complementation , saccharomyces cerevisiae , sequence analysis , nucleic acid sequence , gene , genetics , peptide sequence , homology (biology) , accession number (library science) , sequence alignment , microbiology and biotechnology , mutant
The ZbTPI 1 gene encoding triose phosphate isomerase (TIM) was cloned from a Zygosaccharomyces bailii genomic library by complementation of the Saccharomyces cerevisiae tpi1 mutant strain. The nucleotide sequence of a 1.5 kb fragment showed an open reading frame (ORF) of 746 bp, encoding a protein of 248 amino acid residues. The deduced amino acid sequence shares a high degree of homology with TIMs from other yeast species, including some highly conserved regions. The analysis of the promoter sequence of the ZbTPI1 revealed the presence of putative motifs known to have regulatory functions in S. cerevisiae . The GenBank Accession No. of ZbTPI1 is AF325852. Copyright © 2001 John Wiley & Sons, Ltd.