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Cloning of glycerol‐3‐phosphate dehydrogenase genes ( ZrGPD1 and ZrGPD2 ) and glycerol dehydrogenase genes ( ZrGCY1 and ZrGCY2 ) from the salt‐tolerant yeast Zygosaccharomyces rouxii
Author(s) -
Iwaki Tomoko,
Kurono Sachko,
Yokose Yuki,
Kubota Kenji,
Tamai Youichi,
Watanabe Yasuo
Publication year - 2001
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.722
Subject(s) - biology , gene , saccharomyces cerevisiae , dehydrogenase , yeast , glycerol 3 phosphate dehydrogenase , cloning (programming) , biochemistry , genbank , molecular cloning , glycerol , complementary dna , microbiology and biotechnology , enzyme , computer science , programming language
The ZrGPD1 and ZrGPD2 genes encoding putative glycerol‐3‐phosphate dehydrogenases were isolated from the salt‐tolerant yeast, Zygosaccharomyces rouxii . Both genes are homologous to GPD1 of Saccharomyces cerevisiae and are constitutively expressed in Z. rouxii cells. Putative glycerol dehydrogenase genes, ZrGCY1 and ZrGCY2 , which are highly homologous to GCY1 of S. cerevisiae , were also isolated. Since the level of transcripts of ZrGCY1 and ZrGCY2 increased in Z. rouxii cells subjected to salt stress, it is suggested that the pathway of the signal transduction of salt stress controls the expression of these genes. The Accession Nos of these sequences in GenBank are as follows: ZrGPD1 , AB047394; ZrGPD2 , AB047395; ZrGCY1 , AB047396; ZrGCY2 , AB047397. Copyright © 2001 John Wiley & Sons, Ltd.