Premium
Regulation of the expression of endopolygalacturonase gene PGU1 in Saccharomyces
Author(s) -
Gognies S.,
Simon G.,
Belarbi A.
Publication year - 2001
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.693
Subject(s) - pectinase , biology , saccharomyces cerevisiae , pectin , gene , yeast , biochemistry , gene expression , promoter , saccharomyces , gene cluster , enzyme
Previous work in our laboratory has shown that Saccharomyces bayanus strain SCPP is the only reported yeast expressing the three types of pectolytic enzymes: pectin esterases, pectin lyases and polygalacturonases. One of these enzymes, the endopolygalacturonase (endoPG), hydrolyses plant‐specific polysaccharide pectin. The endoPG encoding gene ( PGU1 ) is also present in Saccharomyces cerevisiae . It has been shown that this endoPG is required for the development of pseudohyphae. Using genomic DNA, the PGU1‐1 and PGU1‐2 promoters of these strains have been amplified and used to construct gene fusions with the β ‐ galactosidase gene. On the basis of β‐galactosidase measurements, we compared the expression of both promoters in different environmental conditions in order to identify their modulation. We have shown that the PGU1 gene is upregulated by the presence of the pectin and the product resulting from endopolygalacturonase activity. Moreover, expression of the PGU1 is also enhanced under respiratory and filament formation conditions. Copyright © 2001 John Wiley & Sons, Ltd.