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Construction of FLAG and histidine tagging vectors for Schizosaccharomyces pombe
Author(s) -
Huang Ying,
Hamada Mitsuhiro,
Patel Jimit,
Maraia Richard J.
Publication year - 2001
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.692
Subject(s) - schizosaccharomyces pombe , biology , protein subunit , fusion protein , histidine , schizosaccharomyces , computational biology , gene , genetics , microbiology and biotechnology , amino acid , saccharomyces cerevisiae , recombinant dna
Schizosaccharomyces pombe is becoming an increasingly popular model system for investigating important cellular processes. To facilitate detection, purification and functional studies of Sz. pombe gene products, we constructed two tagging expression vectors for use in Sz. pombe . These vectors allow proteins to be expressed ectopically as fusion proteins with a FLAG epitope and six histidine residue tags attached to their N‐terminus or C‐terminus. The function and applicability of these vectors were examined and the results are shown using the N‐terminal tagging vector encoding Sfc6p, a subunit of the Sz. pombe RNA polymerase III general transcription factor, TFIIIC. Copyright © 2001 John Wiley & Sons, Ltd.