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Hypoosmolality impedes cytoophidium integrity during nitrogen starvation
Author(s) -
Li YiLan,
Liu JiLong
Publication year - 2021
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.3542
Subject(s) - schizosaccharomyces pombe , biology , saccharomyces cerevisiae , yeast , budding yeast , schizosaccharomyces , fragmentation (computing) , hypha , microbiology and biotechnology , genetics , ecology
CTP synthase (CTPS) cytoophidia have been found in many species over domains of life in the past 10 years, implying the evolutionary conservation of these structures. However, there are differences in cytoophidia between species. The difference in CTPS cytoophidium properties between budding yeast ( Saccharomyces cerevisiae ) and fission yeast ( Schizosaccharomyces pombe ) inspires this research. We study the effects of culture environment on cytoophidia in S. cerevisiae by switching to the optimal medium for S. pombe . S. cerevisiae CTPS cytoophidium fragmentation and pseudohyphae formation are observed after treatment with S. pombe medium YES instead of S. cerevisiae medium YPD. By modifying the level of each ingredient of the media, we find that hypoosmolality impedes cytoophidium integrity during nitrogen starvation. Our study demonstrates the relationship between cytoophidium integrity and environmental stress, supporting the role of cytoophidia in stress resistance.