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Genome editing in the yeast Nakaseomyces delphensis and description of its complete sexual cycle
Author(s) -
Zhou Li Youfang,
Boisnard Stéphanie,
EnacheAngoulvant Adela,
Fairhead Cécile
Publication year - 2021
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.3522
Subject(s) - biology , ploidy , mating type , mating of yeast , genetics , gene , meiosis , mating , saccharomyces cerevisiae , genome , sexual reproduction , yeast , chromosome , strain (injury) , anatomy
Abstract The environmental yeast Nakaseomyces delphensis is, phylogenetically, the closest known species to Candida glabrata , a major fungal pathogen of humans. C. glabrata is haploid and described as asexual, while N. delphensis is also haploid, but has been described as competent for mating and meiosis. Both genomes contain homologues of all the genes necessary for sexual reproduction and also the genes for Ho‐dependent mating‐type switching, like Saccharomyces cerevisiae . We first report the construction of genetically engineered strains of N. delphensis , including by CRISPR‐Cas 9 gene editing. We also report the description of the sexual cycle of N. delphensis. We show that it undergoes Ho‐dependent mating‐type switching in culture and that deletion of the HO gene prevents such switching and allows maintenance of stable, separate, MATa and MATalpha haploid strains. Rare, genetically selected diploids can be obtained through mating of haploid strains, mutated or not for the HO gene. In contrast to HO/HO diploids, which behave as expected, Δho / Δho diploids exhibit unusual profiles in flow cytometry. Both types of diploids can produce recombined haploid cells, which grow like the original haploid‐type strain. Our experiments thus allow the genetic manipulation of N. delphensis and the reconstruction, in the laboratory, of its entire life cycle.