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A history of genome editing in Saccharomyces cerevisiae
Author(s) -
Alexander William G.
Publication year - 2018
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.3300
Subject(s) - genome , biology , genome editing , saccharomyces cerevisiae , budding yeast , computational biology , synthetic biology , model organism , genetics , genome engineering , crispr , yeast , gene
Genome editing is a form of highly precise genetic engineering which produces alterations to an organism's genome as small as a single base pair with no incidental or auxiliary modifications; this technique is crucial to the field of synthetic biology, which requires such precision in the installation of novel genetic circuits into host genomes. While a new methodology for most organisms, genome editing capabilities have been used in the budding yeast Saccharomyces cerevisiae for decades. In this review, I will present a brief history of genome editing in S. cerevisiae , discuss the current gold standard method of Cas9‐mediated genome editing, and speculate on future directions of the field.