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The inheritance of mitochondrial DNA in interspecific Saccharomyces hybrids and their properties in winemaking
Author(s) -
Verspohl Alexandra,
Pignedoli Samuele,
Giudici Paolo
Publication year - 2018
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.3288
Subject(s) - biology , heterosis , mitochondrial dna , heteroplasmy , saccharomyces cerevisiae , hybrid , mating , genetics , introgression , mating type , inheritance (genetic algorithm) , interspecific competition , mating design , yeast , strain (injury) , botany , gene , anatomy
The inheritance of mitochondrial DNA (mtDNA) in yeast hybrids is heteroplasmic, turning into homoplasmic after few cell cycles. Mitotype restoration is reported to be biparental and random. This study shows that mitotype restoration follows fixed schemata. We created isogenic, interspecific Saccharomyces cerevisiae × Saccharomyces uvarum hybrids through direct mating and analysed their mating frequency and mitotype. The mating frequency increased for most crosses in staggered mating. Mitotyping revealed that breeding lines with the same parental strain of S. cerevisiae and different parental strains of S. uvarum give rise to the same mitotype. According to our results, we postulate that the inheritance of mtDNA is dominated by one parental strain and that the superior mitotype is specific to each breeding line. Technological tests showed typical hybrid heterosis. Copyright © 2017 John Wiley & Sons, Ltd.