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Opi1p translocation to the nucleus is regulated by hydrogen peroxide in Saccharomyces cerevisiae
Author(s) -
Camelo Carolina,
VilasBoas Filipe,
Cepeda Andreia Pereira,
Real Carla,
BarrosMartins Joana,
Pinto Francisco,
Soares Helena,
Marinho H. Susana,
Cyrne Luisa
Publication year - 2017
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.3240
Subject(s) - biology , saccharomyces cerevisiae , chromosomal translocation , hydrogen peroxide , nucleus , saccharomyces , microbiology and biotechnology , yeast , genetics , biochemistry , gene
During exposure of yeast cells to low levels of hydrogen peroxide (H 2 O 2 ), the expression of several genes is regulated for cells to adapt to the surrounding oxidative environment. Such adaptation involves modification of plasma membrane lipid composition, reorganization of ergosterol‐rich microdomains and altered gene expression of proteins involved in lipid and vesicle traffic, to decrease permeability to exogenous H 2 O 2 . Opi1p is a transcriptional repressor that is inactive when present at the nuclear membrane/endoplasmic reticulum, but represseses transcription of inositol upstream activating sequence (UAS INO )‐containing genes, many of which are involved in the synthesis of phospholipids and fatty acids, when it is translocated to the nucleus. We investigated whether H 2 O 2 in concentrations inducing adaptation regulates Opi1p function. We found that, in the presence of H 2 O 2 , GFP–Opi1p fusion protein translocates to the nucleus and, concomitantly, the expression of UAS INO ‐containing genes is affected. We also investigated whether cysteine residues of Opi1p were implicated in the H 2 O 2 ‐mediated translocation of this protein to the nucleus and identified cysteine residue 159 as essential for this process. Our work shows that Opi1p is redox‐regulated and establishes a new mechanism of gene regulation involving Opi1p, which is important for adaptation to H 2 O 2 in yeast cells. Copyright © 2017 John Wiley & Sons, Ltd.