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Analysis of Paracoccidioide s lutzii mitochondria: a proteomic approach
Author(s) -
Casaletti L.,
Lima P. S.,
Oliveira L. N.,
Borges C. L.,
Báo S. N.,
Bailão A. M.,
Soares C. M. A.
Publication year - 2017
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.3225
Subject(s) - biology , mitochondrion , dimorphic fungus , autophagy , organelle , glyoxylate cycle , biochemistry , microbiology and biotechnology , yeast , apoptosis , metabolism
The genus Paracoccidioides is composed of thermal dimorphic fungi, causative agents of paracoccidioidomycosis, one of the most frequent systemic mycoses in Latin America. Mitochondria have sophisticated machinery for ATP production, which involves metabolic pathways such as citric acid and glyoxylate cycles, electron transport chain and oxidative phosphorylation. In addition, this organelle performs a variety of functions in the cell, working as an exceptional metabolic signalling centre that contributes to cellular stress responses, as autophagy and apoptosis in eukaryotic organisms. The aim of this work was to perform a descriptive proteomic analysis of mitochondria in Paracoccidioides lutzii yeast cells. After mitochondria fractionation, samples enriched in mitochondrial proteins were digested with trypsin and analysed using a NanoUPLC‐MS E system (Waters Corporation, Manchester, UK). Ours results revealed that the established protocol for purification of mitochondria was very effective for P. lutzii , and 298 proteins were identified as primarily mitochondrial, in our analysis. To our knowledge, this is the first compilation of mitochondrial proteins from P. lutzii , to date. Copyright © 2016 John Wiley & Sons, Ltd.