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Arp2/3 complex and Mps3 are required for regulation of ribosome biosynthesis in the secretory stress response
Author(s) -
Yabuki Yukari,
Katayama Masako,
Kodama Yushi,
Sakamoto Akiko,
Yatsuhashi Ayumi,
Funato Kouichi,
Mizuta Keiko
Publication year - 2017
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.3221
Subject(s) - biology , microbiology and biotechnology , ribosome biogenesis , spindle pole body , ribosome , spindle apparatus , secretory protein , secretion , rna , biochemistry , gene , cell division , cell
Secretory defects cause transcriptional repression of ribosome biogenesis in Saccharomyces cerevisiae . However, the molecular mechanism underlying secretory defect‐induced transcriptional repression of ribosome biogenesis remains to be fully elucidated. In this study, we demonstrated that the Arp2/3 complex was required for reduction of ribosome protein gene expression in response to defective secretion by addition of tunicamycin. Two cmd1 mutants, cmd1‐228 and cmd1‐239 that cause mislocalization of calmodulin and defective mitotic spindle formation, respectively, failed to interact with Arc35, a component of the Arp2/3 complex. These mutants also caused defects in the reduction of ribosome protein gene expression induced by secretory blockade. A mutation in TUB4 ( tub4‐1 ), whose product has an essential function in microtubule organization, showed a similar response. In addition, we showed that the response to a secretory defect required SUN protein Mps3, which was localized at the nuclear envelope and involved in spindle pole body assembly. These results suggest that the Arp2/3 complex is required to transmit signals resulting from secretory blockade, and that the spindle pole body functions as a transit point from cytoplasm to Mps3 at the nuclear envelope. Copyright © 2016 John Wiley & Sons, Ltd.