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Spatial organization of the Schizosaccharomyces pombe genome within the nucleus
Author(s) -
Matsuda Atsushi,
Asakawa Haruhiko,
Haraguchi Tokuko,
Hiraoka Yasushi
Publication year - 2017
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.3217
Subject(s) - schizosaccharomyces pombe , biology , genome , chromosome , schizosaccharomyces , chromatin , nucleus , genomic organization , genetics , model organism , chromosome conformation capture , computational biology , yeast , evolutionary biology , microbiology and biotechnology , saccharomyces cerevisiae , gene , gene expression , enhancer
The fission yeast Schizosaccharomyces pombe is a useful experimental system for studying the organization of chromosomes within the cell nucleus. S. pombe has a small genome that is organized into three chromosomes. The small size of the genome and the small number of chromosomes are advantageous for cytological and genome‐wide studies of chromosomes; however, the small size of the nucleus impedes microscopic observations owing to limits in spatial resolution during imaging. Recent advances in microscopy, such as super‐resolution microscopy, have greatly expanded the use of S. pombe as a model organism in a wide range of studies. In addition, biochemical studies, such as chromatin immunoprecipitation and chromosome conformation capture, have provided complementary approaches. Here, we review the spatial organization of the S. pombe genome as determined by a combination of cytological and biochemical studies. Copyright © 2016 John Wiley & Sons, Ltd.