Premium
IV . Yeast sequencing reports. GUF1 , a gene encoding a novel evolutionarily conserved gtpase in budding yeast
Author(s) -
Kiser Gretchen L.,
Weinert Ted A.
Publication year - 1995
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.320111312
Subject(s) - biology , genetics , gene , caenorhabditis elegans , gtpase , mutant , saccharomyces cerevisiae , yeast
While sequencing a region of chromosome IV adjacent to the checkpoint gene MEC3 , we identified a gene we call GUF1 ( G TPase of U nknown F unction), which predicts a 586 amino acid GTPase of the elongation factor‐type class. The predicted Guf1p protein bears striking sequence similarity to both LepA from Escherichia coli (43% identical) and LK1236·1 from Caenorhabditis elegans (42% identical). Analysis of both a guf1 δ deletion and a putative constitutive‐activating mutant ( GUF1HG ) revealed that GUF1 is not essential nor did mutant cells reveal any marked phenotype. The GenBank Accession Number for the GUF1 sequence is U22360.