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Yeast sequencing reports. Isolation of phosphoribosylpyrophosphate synthetase ( PRS1 ) gene from Candida albicans
Author(s) -
Payne Tracie L.,
Calderone Richard A.
Publication year - 1995
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.320111310
Subject(s) - biology , ecori , gene , candida albicans , homology (biology) , genetics , saccharomyces cerevisiae , microbiology and biotechnology , amino acid , peptide sequence , biochemistry , restriction enzyme
Abstract We have isolated a 3·7 kb Eco R1 fragment from a genomic library of Candida albicans which displayed a 65% level of identity with the PRS gene family ( PRS ) of Saccharomyces cerevisiae . The PRS gene encodes a phosphoribosylpyrophosphate (PRPP) synthetase of S. cerevisiae , which catalyses the synthesis of purines, pyrimidines, and amino acids such as histidine and tryptophan. By Northern analyses, we observed that the entire 3·7 kb Eco R1 fragment as well as a 1·1 kb Kpn I‐ Sac I internal fragment of the 3·7 kb Eco R1 fragment hybridized to the same 1.4 kb transcript. An internal 2·6 kb Kpn I fragment was subcloned and sequenced. A deduced sequence of 321 amino acids representing a polypeptide of 35·2 kDa was determined. A FASTA search indicated that the C. albicans PRS (Ca PRS1) had an overall homology at the amino acid level of 91% with the S. cerevisiae PRS3 . Putative transcriptional start and termination sequences as well as a cation‐binding, PRPP synthetase signature sequence were identified. Ca PRS1 was localized to chromosome 2 of the C. albicans genome. Low stringency hybridizations indicates that the organism may possess multiple PRS genes. The function of these genes in nitrogen signaling is discussed. The Ca PRS 1 sequence submitted to the EMBL data library is available under Accession Number U23934.