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XV . Yeast sequencing reports. DNA sequence analysis of a 13 kbp fragment of the left arm of yeast chromosome XV containing seven new open reading frames
Author(s) -
Casamayor Antonio,
Aldea Martí,
Casas Celia,
Herrero Enrique,
Gamo FranciscoJavier,
Lafuente María J.,
Gancedo Carlos,
Ariño Joaquín
Publication year - 1995
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.320111308
Subject(s) - biology , schizosaccharomyces pombe , genetics , open reading frame , orfs , saccharomyces cerevisiae , cosmid , yeast , caenorhabditis elegans , peptide sequence , microbiology and biotechnology , dna , gene
The sequence of a 13 kbp fragment located in the vicinity of the left telomere of chromosome XV (cosmid pEOA179) has been determined. Seven new open reading frames (ORFs) encoding polypeptides longer than 100 residues have been found (AOB629, AOA342, AOC231, AOE555, AOE236, AOA236 and AOE1045). Three of them show no identity with proteins deposited in the data banks. ORF AOB629 (629 amino acids) has some similarity with previously described ferric reductases from Saccharomyces cerevisiae and Schizosaccharomyces pombe . ORF AOA342 encodes a polypeptide reminiscent of dihydroflavonol‐4‐reductases from a number of plant species. AOE236 displays a high level of identity when compared with peroxisomal membrane proteins previously cloned from the methylotrophic yeast Candida boidinii . Finally, AOE1045 encodes a large protein (1045 residues) with some identity with a hypothetical 147 kDa protein identified during the sequencing of Caenorhabditis elegans chromosome 3. The complete nucleotide sequence of the 13 kbp fragment has been deposited at the EMBL data base (Accession Number Z48239).