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DOG R 1 and DOG R 2 : Two genes from Saccharomyces cerevisiae that confer 2‐deoxyglucose resistance when overexpressed
Author(s) -
RandezGil Francisca,
Blasco Amalia,
Prieto Jose Antonio,
Sanz Pascual
Publication year - 1995
Publication title -
yeast
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.923
H-Index - 102
eISSN - 1097-0061
pISSN - 0749-503X
DOI - 10.1002/yea.320111303
Subject(s) - saccharomyces cerevisiae , biology , enzyme , gene , phosphatase , deoxyglucose , phosphate , microbiology and biotechnology , isozyme , inorganic phosphate , biochemistry
Saccharomyces cerevisiae contains two genes ( DOG R 1 and DOG R 2 ) that are able to confer 2‐deoxyglucose resistance when they are overexpressed. These genes are very similar, sharing 92% identity at the protein level. They code for two isoenzymes with 2‐deoxyglucose‐6 phosphate (2‐DOG‐6P) phosphatase activity. These enzymes have been purified and characterized. Dog R 1p shows an optimum pH of 6, an optimum temperature of 30°C and a K M on 2‐DOG‐6P of 17 mM. Dog R 2p shows a similar optimum pH, but the optimum temperature is 40°C and it exhibits a K M on 2‐DOG‐6P of 41 mM. Both enzymes require 10 mM‐MgCl 2 for maximal activity and they are inhibited by inorganic phosphate.